Recommended Readings: Paul Cohen, M.D., Ph.D. June 24

Special Seminar 
Tuesday, June 24, 2014
4:00 p.m., Carson Family Auditorium

Paul Cohen, M.D., Ph.D.
Postdoctoral Fellow, Dana Farber Cancer Institute
Attending Cardiologist, Brigham and Women’s Hospital
Instructor in Medicine, Harvard Medical School

Transcriptional Determinants of Subcutaneous and Visceral Adipose Tissue: Towards Engineering Healthier Fat

Recommended Readings:

Empirical Articles

Cohen, P., Levy, J. D., Zhang, Y., Frontini, A., Kolodin, D. P., Svensson, K. J., … Spiegelman, B. M. (2014). Ablation of PRDM16 and beige adipose causes metabolic dysfunction and a subcutaneous to visceral fat switch. Cell, 156(1-2), 304–16. doi:10.1016/j.cell.2013.12.021

Seale, P., Conroe, H. M., Estall, J., Kajimura, S., Frontini, A., Ishibashi, J., … Spiegelman, B. M. (2011). Prdm16 determines the thermogenic program of subcutaneous white adipose tissue in mice. The Journal of Clinical Investigation, 121(1), 53–56. doi:10.1172/JCI44271.96

Review Papers

Wu, J., Cohen, P., & Spiegelman, B. M. (2013). Adaptive thermogenesis in adipocytes: is beige the new brown? Genes & Development, 27(3), 234–50. doi:10.1101/gad.211649.112

Recommended Readings: Thomas Walz, Ph.D. June 23

Special Seminar 
Monday, June 23, 2014
4:00 p.m., Carson Family Auditorium

Thomas Walz, Ph.D
Professor, Department of Cell Biology
Harvard Medical School
Investigator, Howard Hughes Medical Institute

Using Two-dimensional Crystals of Aquaporin-0 to Investigate Lipid-Protein Interactions

Recommended Readings:

Empirical Articles

Aponte-Santamaría, C., Briones, R., Schenk, A. D., Walz, T., & de Groot, B. L. (2012). Molecular driving forces defining lipid positions around aquaporin-0. Proceedings of the National Academy of Sciences, 109(25), 9887–9892. doi:10.1073/pnas.1121054109

Gonen, T., Cheng, Y., Sliz, P., Hiroaki, Y., Fujiyoshi, Y., Harrison, S. C., & Walz, T. (2005). Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature, 438(7068), 633–638. doi:10.1038/nature04321

Gonen, T., Sliz, P., Kistler, J., Cheng, Y., & Walz, T. (2004). Aquaporin-0 membrane junctions reveal the structure of a closed water pore. Nature, 429(6988), 193–197. doi:10.1038/nature02503

Hite, R. K., Li, Z., & Walz, T. (2010). Principles of membrane protein interactions with annular lipids deduced from aquaporin-0 2D crystals. The EMBO Journal, 29(10), 1652–1658. doi:10.1038/emboj.2010.68

Review Papers

Gonen, T., & Walz, T. (2006). The structure of aquaporins. Quarterly Reviews of Biophysics, 39(4), 361–396. doi:10.1017/S0033583506004458

Raunser, S., & Walz, T. (2009). Electron crystallography as a technique to study the structure on membrane proteins in a lipidic environment. Annual Review of Biophysics, 38, 89–105. doi:10.1146/annurev.biophys.050708.133649

Recommended Readings: Daniel Finley, Ph.D. May 30

Friday Lecture Series
Friday, May 30, 2014
3:45 p.m., Caspary Auditorium

Daniel Finley, Ph.D.
Professor
Department of Cell Biology
Harvard Medical School

Regulation of Proteasome Activity by Ubiquitin Chain Editing

Recommended Readings:

Empirical Articles

Crosas, B., Hanna, J., Kirkpatrick, D. S., Zhang, D. P., Tone, Y., Hathaway, N. a, … Finley, D. (2006). Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell, 127(7), 1401–1413. doi:10.1016/j.cell.2006.09.051

Hanna, J., Hathaway, N. a, Tone, Y., Crosas, B., Elsasser, S., Kirkpatrick, D. S., … Finley, D. (2006). Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Cell, 127(1), 99–111. doi:10.1016/j.cell.2006.07.038

Lee, B.-H., Lee, M. J., Park, S., Oh, D.-C., Elsasser, S., Chen, P.-C., … Finley, D. (2010). Enhancement of proteasome activity by a small-molecule inhibitor of USP14. Nature, 467(7312), 179–184. doi:10.1038/nature09299

Leggett, D. S., Hanna, J., Borodovsky, A., Crosas, B., Schmidt, M., Baker, R. T., … Finley, D. (2002). Multiple associated proteins regulate proteasome structure and function. Molecular Cell, 10(3), 495–507.

Review Papers

Finley, D. (2009). Recognition and Processing of Ubiquitin-Protein Conjugates by the Proteasome. Annual Review of Biochemistry, 78(1), 477–513. doi:10.1146/annurev.biochem.78.081507.101607

Schmidt, M., & Finley, D. (2014). Regulation of proteasome activity in health and disease. Biochimica et Biophysica Acta, 1843(1), 13–25. doi:10.1016/j.bbamcr.2013.08.012

 

Recommended Readings: Craig B. Thompson, M.D. May 23

Friday Lecture Series
Friday, May 23, 2014
3:45 p.m., Caspary Auditorium

Craig B. Thompson, M.D.
President and CEO
Memorial Sloan Kettering Cancer Center

What Cancer Is Teaching Us about Cellular Metabolism

Recommended Readings:

Empirical Papers

Buzzai, M., Bauer, D. E., Jones, R. G., Deberardinis, R. J., Hatzivassiliou, G., Elstrom, R. L., & Thompson, C. B. (2005). The glucose dependence of Akt-transformed cells can be reversed by pharmacologic activation of fatty acid beta-oxidation. Oncogene, 24(26), 4165–4173. doi:10.1038/sj.onc.1208622

Lum, J. J., Bauer, D. E., Kong, M., Harris, M. H., Li, C., Lindsten, T., & Thompson, C. B. (2005). Growth factor regulation of autophagy and cell survival in the absence of apoptosis. Cell, 120(2), 237–248. doi:10.1016/j.cell.2004.11.046

Selak, M. a, Armour, S. M., MacKenzie, E. D., Boulahbel, H., Watson, D. G., Mansfield, K. D., … Gottlieb, E. (2005). Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase. Cancer Cell, 7(1), 77–85. doi:10.1016/j.ccr.2004.11.022

Ye, J., Mancuso, A., Tong, X., Ward, P. S., Fan, J., & Rabinowitz, J. D. (2012). Pyruvate kinase M2 promotes de novo serine synthesis to sustain mTORC1 activity and cell proliferation. Proceedings of the National Academy of Sciences, 109(18), 2–7. doi:10.1073/pnas.1204176109

Review Papers

DeBerardinis, R. J., Lum, J. J., Hatzivassiliou, G., & Thompson, C. B. (2008). The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metabolism, 7(1), 11–20. doi:10.1016/j.cmet.2007.10.002

Vander Heiden, M. G., Cantley, L. C., & Thompson, C. B. (2009). Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science, 324(5930), 1029–1033. doi:10.1126/science.1160809

Ward, P. S., & Thompson, C. B. (2012). Metabolic reprogramming: a cancer hallmark even Warburg did not anticipate. Cancer Cell, 21(3), 297–308. doi:10.1016/j.ccr.2012.02.014

Recommended Readings: Allan C Spradling, Ph.D. May 16

Friday Lecture Series
Friday, May 16, 2014
3:45 p.m., Caspary Auditorium

Allan C Spradling, Ph.D.
Director, Department of Embryology
Carnegie Institution for Science

Investigator, Howard Hughes Medical Institute

Epigenetic and genetic programing of Drosophila development

Recommended Readings:

Empirical Articles

Morris, L. X., & Spradling, A. C. (2011). Long-term live imaging provides new insight into stem cell regulation and germline-soma coordination in the Drosophila ovary. Development, 138(11), 2207–2215. doi:10.1242/dev.065508

Nystul, T., & Spradling, A. (2007). An epithelial niche in the Drosophila ovary undergoes long-range stem cell replacement. Cell Stem Cell, 1(3), 277–285. doi:10.1016/j.stem.2007.07.009

Skora, A. D., & Spradling, A. C. (2010). Epigenetic stability increases extensively during Drosophila follicle stem cell differentiation. Proceedings of the National Academy of Sciences, 107(16), 7389–7394. doi:10.1073/pnas.1003180107

Xie, T., & Spradling, A. C. (2000). A niche maintaining germ line stem cells in the Drosophila ovary. Science, 290(5490), 328–330. doi:10.1126/science.290.5490.328

Review Papers

Losick, V. P., Morris, L. X., Fox, D. T., & Spradling, A. (2011). Drosophila stem cell niches: a decade of discovery suggests a unified view of stem cell regulation. Developmental Cell, 21(1), 159–171. doi:10.1016/j.devcel.2011.06.018

Morrison, S. J., & Spradling, A. C. (2008). Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. Cell, 132(4), 598–611. doi:10.1016/j.cell.2008.01.038

Recommended Readings: Sangeeta Bhatia, M.D., Ph.D. May 12

SPECIAL SEMINAR
Monday, May 12, 2014
4:00 p.m., Carson Family Auditorium

Sangeeta Bhatia, M.D., Ph.D.
John J. and Dorothy Wilson Professor of Health Sciences & Technology
Institute for Medical Engineering and Science
Director, Laboratory for Multiscale Regenerative Technologies
Massachusetts Institute of Technology
Investigator, Howard Hughes Medical Institute

Tiny Technologies for Studying Tissue Microenvironments

Recommended Readings:

Empirical Papers

Chen, A., & Thomas, D. (2011). Humanized mice with ectopic artificial liver tissues. Proceedings of the National Academy of Sciences, 108(29), 11842–11847. doi:10.1073/pnas.1101791108

Hui, E. E., & Bhatia, S. N. (2007). Micromechanical control of cell-cell interactions. Proceedings of the National Academy of Sciences, 104(14), 5722–5726. doi:10.1073/pnas.0608660104

Khetani, S. R., & Bhatia, S. N. (2008). Microscale culture of human liver cells for drug development. Nature Biotechnology, 26(1), 120–126. doi:10.1038/nbt1361

Review Papers

Underhill, G. H., Galie, P., Chen, C. S., & Bhatia, S. N. (2012). Bioengineering methods for analysis of cells in vitro. Annual Review of Cell and Developmental Biology, 28, 385–410. doi:10.1146/annurev-cellbio-101011-155709

Wong, I. Y., Bhatia, S. N., & Toner, M. (2013). Nanotechnology: emerging tools for biology and medicine. Genes & Development, 27(22), 2397–2408. doi:10.1101/gad.226837.113

Recommended Readings: Robert Lefkowitz, M.D. May 9

Friday Lecture Series
Friday, May 9, 2014
3:45 p.m., Carson Family Auditorium

Robert Lefkowitz, M.D.
James B. Duke Professor of Medicine
Professor of Biochemistry
Duke University
Investigator, Howard Hughes Medical Institute

Seven Transmembrane Receptors

Recommended Readings:

Empirical Articles

Hara, M. R., Kovacs, J. J., Whalen, E. J., Rajagopal, S., Strachan, R. T., Grant, W., … Lefkowitz, R. J. (2011). A stress response pathway regulates DNA damage through β2-adrenoreceptors and β-arrestin-1. Nature, 477(7364), 349–353. doi:10.1038/nature10368

Kahsai, A. W., Xiao, K., Rajagopal, S., Ahn, S., Shukla, A. K., Sun, J., … Lefkowitz, R. J. (2011). Multiple ligand-specific conformations of the β2-adrenergic receptor. Nature Chemical Biology, 7(10), 692–700. doi:10.1038/nchembio.634

Rajagopal, S., Ahn, S., Rominger, D. H., Gowen-macdonald, W., Lam, C. M., Dewire, S. M., … Lefkowitz, R. J. (2011). Quantifying ligand bias at seven-transmembrane receptors. Molecular Pharmacology, 80(3), 367–3. doi:10.1124/mol.111.072801.

Shukla, A. K., Manglik, A., Kruse, A. C., Xiao, K., Reis, R. I., Tseng, W.-C., … Lefkowitz, R. J. (2013). Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide. Nature, 497(7447), 137–141. doi:10.1038/nature12120

Review Papers

DeWire, S. M., Ahn, S., Lefkowitz, R. J., & Shenoy, S. K. (2007). β-Arrestins and Cell Signaling. Annual Review of Physiology, 69(1), 483–510. doi:10.1146/annurev.physiol.69.022405.154749

Lefkowitz, R. J., & Shenoy, S. K. (2005). Transduction of receptor signals by beta-arrestins. Science, 308(5721), 512–517. doi:10.1126/science.1109237

Pierce, K. L., Premont, R. T., & Lefkowitz, R. J. (2002). Seven-transmembrane receptors. Nature Reviews Molecular Cell Biology, 3(9), 639–50. doi:10.1038/nrm908

Wisler, J. W., Xiao, K., Thomsen, A. R., & Lefkowitz, R. J. (2014). Recent developments in biased agonism. Current Opinion in Cell Biology, 27, 18–24. doi:10.1016/j.ceb.2013.10.008

Recommended Readings: David Kingsley, Ph.D.

Friday Lecture Series

The Fairfield Osborn Memorial Lecture

Fishing for the Secrets of Vertebrate Evolution

David Kingsley, Ph.D., professor, developmental biology, Stanford University;

investigator, Howard Hughes Medical Institute

April 18, 2014

3:45 p.m.-5:00 p.m. (Refreshments, 3:15 p.m., Abby Lounge)

Caspary Auditorium

Recommended Readings

han, Y. F., Marks, M. E., Jones, F. C., Villarreal Jr., G., Shapiro, M. D., Brady, S. D., . . . Kingsley, D. M. (2010). Adaptive evolution of pelvic reduction in sticklebacks by recurrent deletion of a pitxl enhancer. Science, 327(5963), 302-305

Haussler, D., O’Brien, S. J., Ryder, O. A., Keith Barker, F., Clamp, M., Crawford, A. J., . . . Turner, S. (2009). Genome 10K: A proposal to obtain whole-genome sequence for 10000 vertebrate species. Journal of Heredity, 100(6), 659-674

Jones, F. C., Grabherr, M. G., Chan, Y. F., Russell, P., Mauceli, E., Johnson, J., . . . Kingsley, D. M. (2012). The genomic basis of adaptive evolution in threespine sticklebacks. Nature, 484(7392), 55-61

Kingsley, D. M., Zhu, B., Osoegawa, K., De Jong, P. J., Schein, J., Marra, M., . . . Myers, R. (2004). New genomic tools for molecular studies of evolutionary change in threespine sticklebacks. Behaviour, 141(11-12), 1331-1344

Lowe, C. B., Kellis, M., Siepel, A., Raney, B. J., Clamp, M., Salama, S. R., . . . Haussler, D. (2011). Three periods of regulatory innovation during vertebrate evolution. Science, 333(6045), 1019-1024

Shapiro, M. D., Bell, M. A., & Kingsley, D. M. (2006). Parallel genetic origins of pelvic reduction in vertebrates. Proceedings of the National Academy of Sciences of the United States of America, 103(37), 13753-13758

 

Recommended Readings: Hermann Steller, Ph.D May 5

Monday Lecture Series
Monday, May 5, 2014
4:00 p.m., Carson Family Auditorium

Hermann Steller, Ph.D
Strang Professor, Strang Laboratory of Apoptosis and Cancer Biology
The Rockefeller University
Investigator, Howard Hughes Medical Institute

Regulation of Protein Degradation in Development, Aging and Disease

Recommended Readings:

Empirical Articles

Bader, M., Benjamin, S., Wapinski, O. L., Smith, D. M., Goldberg, A. L., & Steller, H. (2011). A conserved F box regulatory complex controls proteasome activity in Drosophila. Cell, 145(3), 371–382. doi:10.1016/j.cell.2011.03.021

Cho-Park, P. F., & Steller, H. (2013). Proteasome regulation by ADP-ribosylation. Cell, 153(3), 614–627. doi:10.1016/j.cell.2013.03.040

Review Papers

Finley, D. (2009). Recognition and processing of ubiquitin-protein conjugates by the proteasome. Annual Review of Biochemistry, 78(1), 477–513. doi:10.1146/annurev.biochem.78.081507.101607

Glickman, M. H., & Ciechanover, A. (2002). The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiological Reviews, 82(2), 373–428. doi:10.1152/physrev.00027.2001

Murata, S., Yashiroda, H., & Tanaka, K. (2009). Molecular mechanisms of proteasome assembly. Nature Reviews Molecular Cell Biology, 10(2), 104–15. doi:10.1038/nrm2630

Recommended Readings: Mark M. Davis, Ph.D. April 25

Friday Lecture Series
Friday, April 25, 2014
3:45 p.m., Caspary Auditorium

Mark M. Davis, Ph.D.
Burt and Marion Avery Family Professor of Immunology
Director, Stanford Institute for Immunity, Transplantation and Infection
Member, Bio-X and Stanford Cancer Institute
Stanford University

Reinventing Human Immunology

Recommended Readings:

Davis, M. M. (2008). A prescription for human immunology. Immunity, 29(6), 835–838. doi:10.1016/j.immuni.2008.12.003

Davis, M. M. (2012). Immunology taught by humans. Science Translational Medicine, 4(117), 117fs2. doi:10.1126/scitranslmed.3003385

Kidd, B. a, Peters, L. a, Schadt, E. E., & Dudley, J. T. (2014). Unifying immunology with informatics and multiscale biology. Nature Immunology, 15(2), 118–127. doi:10.1038/ni.2787

Maecker, H. T., McCoy, J. P., & Nussenblatt, R. (2012). Standardizing immunophenotyping for the Human Immunology Project. Nature Reviews Immunology, 12(3), 191–200. doi:10.1038/nri3158

Su, L. F., Han, A., McGuire, H. M., Furman, D., Newell, E. W., & Davis, M. M. (2014). The Promised Land of Human Immunology. Cold Spring Harbor Symposia on Quantitative Biology. doi:10.1101/sqb.2013.78.022905